How are Stem Cells Prepared and Administered?

This is approximately a 2½ – 3 hour outpatient procedure and the differences between this and other Stem Cell procedures are:

• Less painful than a bone marrow transplant
• More Adult Stem Cells per unit volume can be harvested
• No ethical orr moral issues in harvesting adipose (fat) tissue

The process is as follows:

Step 1

Before harvesting the adipose (fat) tissue, it is essential that a sterilised canister is used and filled with 500mL of washing solutions

Step 2

In order to prepare plasma, blood is collected from the patient (see PRP Preparation Protocol)

Step 3

Adipose tissue is then harvested from the patient via tumescent liposuction

Step 4

The adipose collected is then transferred into falcon tubes and then put in to a 37ºC shaking waterbath

Step 5

These tubes tubes are then centrifuged

Step 6

After the centrifuging process, a stem cell pallet will form on the bottom of each falcon tube. Using a pipette and taking extra care not the stir the contents, the pallets are then carefully removed from each tube.

Step 7

The pallets are then first filtered

Step 8

Using a syringe, we collect a sample from the tube for cell counting and staining for viability.

Step 9

The remaining stem cells are then activated using the AdiLight

Step 10

The cells are then administered back to the patient through one or more of the following modes of administration

• Intravenous: Administered through a standard intravenous drip

• Injected: Administered directly into a localised area