Adipose Tissue Derived Stem Cells
What is Adipose Tissue?
Adipose tissue is simply your body fat. This fat is the primary storage site for triglycerides. Adipose tissue is common in 2 forms. white adipose tissue (which is the most common) and brown adipose tissue.
White adipose tissue serves three functions: heat insulation, mechanical cushion, and most importantly, a source of energy.
In the past 15 years, researchers have found body fat to contain large amounts of stem cells and many other cell strands which are beneficial to our bodies.
How do we obtain the Adipose Tissue?
We will extract the adipose (fat) tissue using a mini-liposuction procedure while the area is treated with local anaesthesia. During this process we will extract approximately 300 cc’s of adipose tissue, which will then be treated with ultrasonic cavitation to purify and refine the valuable regenerative cells.
We expect the collection of your body fat will take approximately 1 to 1.5 hours.
What is the Process of Cell Seperation?
Over the many years we have been working to find a cleaner way seperating the cellular mix from the body fat. Many other companies still use the initial separation methods of Enzymes & Chemicals…
WE DO NOT!
We have developed our own patented method of “Ultrasonic cavitation”, a process which involves no added enzymes or chemicals which means it’s cleaner and safer. During ultrasonic cavitation the fat cells are mechanically broken down and the cellular mix separated away from the bio-scaffold and connecting collagen. Leaving behind purified cells which include stem cells, progenitor and other immune modulating cells. This mix of valuable regenerative cells is known as Stromal Vascular Fraction (SVF) cells, sometimes referred to as stromal cells. About 60% of these SVF cells will be pure stem cells while the remainder will be a variation of other regenerative and growth cells. Before the cells are re-injected into your body during the final part of the process we will perform a quality test using an instrument called a flow cytometer which will examine the cell viability and count.
How do these cells function?
Stem cells are cells that can differentiate into other types of tissue to restore function and reduce pain. We used to believe that once a stem cell selects a specialised purpose it can no longer differentiate into other parts of the body. However, scientists have worked out how to make the body reverse this and fully differentiated cells can be induced to become stem cells in a test tube. These are called induced Pluripotent Stem Cells (iPSCs). We now believe that all cells can become any other if the circumstances are right. Adult stem cells are found in abundance in adipose (fat) tissue, where more than 5 million stem cells reside in every gram. Our medical doctors can extract cells from your own body in order to provide treatment using your very own cells (a process called autologous therapy).
Perfecting the Stem Cell Treatment
The flow cytometer is required in order to define the optimal formulation and dosage of stem cell products. Our team records cell numbers and viability so that we can gain a better understanding of what constitutes a successful treatment. Although it is not yet possible to predict what number of cells will be recovered, it is very important that we know the total cell count and the viable cell count. It is only with this data that we will begin to understand why treatments are very successful, only slightly successful or not successful at all. Our principal doctor, Dr Bright also investigates advanced ways to preserve the extracellular matrix and improve bio-scaffolding to further perfect treatment.
Before each patients treatment the cells are counted in a flow cytometer and cell viability is measured with live/dead stains.
Each time we make a change to any of our protocols for cell harvesting, separation or storage we test our cells by growing them in culture. This shows that they are alive and able to multiply. To show that they are fully healthy and functional we then get them to grow into bone, cartilage or fat cells (differentiation).
Injecting into a vein and into the joint
We will administer the stem cell treatment with multiple methods.
- Local Injection by ultrasound guidance (alternatively known as intra-articular injection)
- Intravenous Injection (alternatively known as injection into the vein)
We believe this is better because each of these illnesses has a local pathology and a central pathology. The site of injury has been inflamed for a long time (chronic inflammation). The resident stem cell population has been working very hard to repair the damage and over the course of time the stem cells have become worn out and slowly die. This essentially causes a state of ‘stem cell depletion’. Then when we inject our mix of cells, cytokines and bio-scaffold (known as stromed) the cytokines are able to start reversing the inflammation and down-regulating the inflammatory genes immediately. This improves the health of the tissues allowing the cells that have always been there to work better. The stem cells we have implanted will then bring the level of stem cells back to normal. This restores the natural balance and allows the body to heal itself.
Caplan et al, The MSC: An Injury Drugstore, DOI 10.1016/j.stem.2011.06.008
Freezing Stem Cells for Future Use
The TGA allow us to store cells for a prescribed course of treatments. Excess cells not used at the end of the course of treatments will be destroyed.
If you would like your cells stored long term just in case you need them for some unexpected problem then they will need to go to a Stem Cell bank such as Stemlife, Cryosite or Cell Care.
Results Post Treatment
The attached link is a publication for treating osteoarthritis with stromal cells from fat. This study is utilizing the Macquarie Stem Cells cells method of cell separation. It has been published in the Journal Of Evidence Based Medicine & Healthcare Oct. 2015
We have more documents inclusive of MRI and X-ray results post treatment, just scroll down to the bottom of the page and request our information pack.
We have been able to use these cells to treat a variety of issues with great success, further information can be obtained from the links below.
- Osteoarthritis Treatment
- Rheumatoid Arthritis Treatment
- Muscle & Tendon Tears
- Ligament & Cartilage Tears
- Disc Bulges & Herniation
- Neuropathic Pain